Biologist Job at GAP Solutions, Inc., Gaithersburg, MD

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  • GAP Solutions, Inc.
  • Gaithersburg, MD

Job Description

Position Objective: Provide services as a Biologist in support of the overall functions of the Vaccine Research Center (VRC), Vaccine Production Program (VPP) within the National Institute of Allergy and Infectious Diseases (NIAID).

Duties And Responsibilities

  • Contribute to the generation of stable cell lines for vaccine and therapeutic protein candidates including monoclonal antibodies, recombinant proteins, and virus-like particles.
  • Prepare plasmid DNA using standard molecular cloning techniques including PCR, restriction digest, and bacterial culture.
  • Independently execute each step of the cell line development process:
    • Transfection
    • Pool generation
    • Single cell cloning
    • Clone expansion in static culture plates and shake flasks
    • Fed-batch analysis
    • Clone stability studies
  • Use automated platforms for clone selection and screening.
  • Participate in cell line development platform design, optimization, and cycle-time reduction studies including methods for clone selection, expansion, ranking, and characterization.
  • Perform small-scale process development studies using various culture vessels including tissue culture plates, shake flasks, and small-scale bioreactors.
  • Generate stable pools and perform transient protein production for supply of reagent-grade material
  • Independently complete experiments and analyze data with minimal supervision.
  • Provide laboratory support for medium preparation and ordering of supplies.
  • Write and review technical reports documenting process development studies and stable cell line generation work.
  • Work with other team members to generate experimental plans for process development studies.
  • Write and review standard operating procedures for cell line development processes and instrumentation.
  • Maintain complete documentation records, including a detailed and up-to-date laboratory notebook, to support regulatory filings.
  • Coordinate cell line development projects in collaboration with the project lead and Federal Task Leader:
    • Plan experiments to fit within required timelines
    • Communicate any issues or delays promptly and help to identify solutions
    • Maintain cell line development master sheet file with all critical information and data
  • Regularly communicate progress to project lead and present work to cell line development team.
  • Participate in project meetings.
  • Contribute results and studies for publication in peer-reviewed journals.
  • Maintain broad knowledge of state-of-the-art principles.

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